Amperometric measurements of catecholamine release from single vesicles in MN9D cells |
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| Authors: | Yan Dong Michael L Heien Marc M Maxson Andrew G Ewing |
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| Institution: | 1. Department of Chemistry, The Pennsylvania State University, University Park, Pennsylvania, USA;2. Department of Neurosurgery, Changzheng Hospital, Second Military Medical University, Shanghai, China;3. Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, Pennsylvania, USA;4. Department of Neural and Behavioral Sciences, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania, USA;5. Department of Chemistry, G?teborg University, G?teborg, Sweden |
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| Abstract: | MN9D cells have been used as a successful model to investigate dopamine pharmacology and to test the specific effects of drugs for the treatment of Parkinson’s disease. However, quantitative measurements of quantal release from these cells have not been carried out. In this work, we used amperometry to investigate catecholamine release from MN9D cells. Amperometric events were observed in both undifferentiated and differentiated (butyric acid‐treated) cells. An increase in quantal size and half‐width was observed for differentiated cells versus undifferentiated cells; however, the number of events per cell and the amplitude remained constant. In transmission electron microscopy images, no obvious cluster of small synaptic vesicles was observed, and large dense‐core vesicles were present in the cell body of undifferentiated cells; however, after differentiation, vesicles were concentrated in the cell processes. In differentiated cells, l ‐DOPA caused an increase in quantal size and half‐width, which could be blocked by the vesicular monoamine transporter inhibitor, reserpine. |
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| Keywords: | catecholamine dopamine half‐width MD9D cells Parkinson’ s disease quantal size |
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