Solubilization of Active Brain α1-Adrenoceptors by a Zwitterionic Detergent |
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Authors: | Pascale Guicheney Alain Rappaport Dominique Marcel |
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Institution: | U7 INSERMILA 3/8 CNRS, Department of Pharmacology, Hôpital Necker, Paris, France |
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Abstract: | Solubilization of rat brain alpha 1-adrenoceptors was performed by treatment with 6 mM CHAPS (3-(3-cholamidopropyl)dimethylammonio] - 1 - propanesulfonate). The alpha 1-adrenoceptor antagonist 3H]prazosin was shown to bind reversibly and specifically to the soluble extract obtained after centrifugation at 150,000 X g for 1 h. Separation of the soluble 3H]prazosin-bound complexes was performed by the polyethylene glycol precipitation technique followed by filtration. A Scatchard plot of the concentration-dependent binding curve showed only one class of binding sites, with a high affinity for 3H]prazosin. Affinity of the solubilized receptors for the ligand increased as the CHAPS concentration in the assay medium decreased; the number of binding sites remained unchanged (approximately equal to 70 fmol/mg protein). This corresponds to a 30% recovery of original membrane sites. The solubilized receptors presented the same characteristics of specificity and stereospecificity as membrane alpha 1-adrenoceptors. Moreover, 150 mM NaCl was found to modulate the affinity of epinephrine for the 3H]prazosin-bound soluble complex, as previously described for membrane preparations. Thus, CHAPS appears to be a suitable detergent for solubilizing rat brain alpha 1-adrenoceptors and preserving their functional activities. |
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Keywords: | Solubilization Zwitterionic detergent Brain α1-adrenoceptors [3H] Prazosin |
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