Effects of Detergents, Proteins, and Lipids on 2':3'-Cyclic-Nucleotide 3'-Phosphodiesterase Activity |
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| Authors: | Patric A Clapshaw Wilfried Seifert |
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| Institution: | Friendrich-Miescher-Laboratorium der Max-Planck-Gesellschaft, Spemannstraβe 37–39. 7400 Tübingen. Federal Republic of Germany |
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| Abstract: | The myelin marker 2':3'-cyclic-nucleotide 34'-phosphodiesterase (CNPase) was isolated to a lipid- and phosphate-free stage. The effects of exogenously added lipids were tested on this preparation and compared to the known stimulation of the enzyme by detergents and proteins. CNPase could be stimulated 2-3 fold by these various agents which appeared to be additive in their effect. Enzyme-protein and enzyme-lipid interactions and possible medical use of the improved assay conditions for CNPase employed in the study are discussed. |
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| Keywords: | Membrane enzyme Cyclic nucleotides Lipid-protein interaction Multiple sclerosis |
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