Role of GPI-anchored NCAM-120 in rabies virus infection |
| |
Authors: | Hotta Kozue Motoi Yurie Okutani Akiko Kaku Yoshihiro Noguchi Akira Inoue Satoshi Yamada Akio |
| |
Institution: | The United Graduate school of Veterinary Science, Gifu University, Ynagido 1-1, Gifu 501-1193, Japan. |
| |
Abstract: | Although the neural cell adhesion molecule (NCAM) -140 and -180 have been shown to serve as a receptor for rabies virus (RV), it was not known whether the other major isoform of NCAM, GPI-anchored NCAM-120 functions as RV receptor. In this study, we have established HEp-2 cells stably expressing NCAM-120 or NCAM-140, and their susceptibilities to RV infection were compared. The results demonstrated that NCAM-120 served as virus attachment protein; however, the cells expressing NCAM-120 did not support efficient RV replication. Furthermore, the level of IFN-ss mRNA was apparently elevated in NCAM-120 expressing cells but not in NCAM-140 expressing cells, suggesting that GPI-anchored NCAM-120 suppressed RV replication via induction of IFN-ss even though NCAM-120 was able to promote virus penetration into the cells. |
| |
Keywords: | |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|