不同周龄BWEL-SPF种鸡生理生化指标的测定

目的测定不同周龄的BWEL-SPF种鸡生理和生化指标，并对测定值进行t-检验。方法分别采用计数板法、血涂片法、血液全自动分析仪测定法、双缩尿法、溴甲酚绿法、酶比色法和硫氰酸汞比色法等测定了10、40和60周龄的雌、雄BWEL-SPF种鸡的生理生化指标，并对雌、雄差异进行了统计学分析。结果在所测定的生理生化指标中，不同周龄雌雄之间差异显著性表现项目不同，其中在10周龄时，只有总胆固醇（CHO）在雌、雄之间差异极显著（P〈0．01）；40周龄时，甘油三酯（TG）、在雌、雄之间差异极显著（P〈0．01）；总胆固醇（CHO）为雌、雄之间差异显著（P〈0．05）；60周龄时，出血时间（BT）、嗜碱性粒细胞（B）、单核细胞（M）、谷丙转氨酶（ALT）、葡萄糖（Glu）在雌、雄之间差异极显著（P〈0．01），而凝血时间（CT）和甘油三酯（TG）在雌、雄之间差异显著（P〈0．05）．除此以外，其它生理生化指标在雌雄之间差异不显著。结论BWEL-SPF种鸡的生理和生化指标随周龄不同，在雌、雄之间的差异随之变化。

Determination of Values of Physiological and Biochemical Parameters in BWEL-SPF Chickens at Age of 10,40 and 60 Weeks

Objective To determine the values of physiological and biochemical parameters in BWEL-SPF chickens at age of 10,40 and 60 weeks. Methods The values of T,P, BT,CT, ESR,systolic pressure,diastolic pressure,RBC,HCT,MCV, RDW,HGB,MCH,MCHC,PLT,MPV,WBC,N,E,B,L,M,TP,AIb,AST,ALT,GIu and TG and CHO in BWEL-SPF chickens at age of 10,40 and 60 weeks, both male and female, were determined by corresponding measurement and t-test was made to analyze the results. Results There was a significant difference between CHO values in male and female chickens at age of 10 weeks （ P 〈 0.01 ）, a very significant difference between TG, Ca, Na and Mg values in male and female chickens at age of 40 weeks （ P 〈 0.01 ） ,a significant difference between CHO values in male and female chickens at age of 40 weeks （ P 〈 0.05） ,a very significant difference between BT, B, M, ALT and Glu values in male and female chickens at age of 60 weeks （P 〈 0.01 ）, and a significant difference between CT and TG values in male and female chickens at age of 60＇weeks （ P 〈 0.05）. Conclusion The values of the physiological and biochemical parameters were different between male and female BWEL-SPF chickens at age of 10,40 and 60 weeks.