一种新的大鼠隐睾模型的建立

目的改善大鼠隐睾模型的制作方法,提高隐睾模型的质量,并对新模型的稳定性进行研究。方法28只大鼠随机分为对照组（ctrl）和模型组（modl）采用模拟失重大鼠模型,对大鼠进行3周尾部悬吊进行造模,随后模型组大鼠解悬吊恢复8周观察该模型的稳定性。结果经过3周的尾部悬吊,模型组所有大鼠睾丸均滑入腹腔,同时和对照组相比,睾丸和附睾的重量出现极显著的降低（P〈0.01）。HE染色发现对照组大鼠睾丸的生精小管结构排列紊乱,精原细胞消失,附睾尾中成熟精子消失。经过8周的恢复,大鼠的睾丸及附睾仍未恢复到正常水平（P〈0.01）,HE染色显示其生精小管结构和精原细胞数量并未出现明显的改善。结论尾吊法所建立的大鼠隐睾模型效果稳定,可以有效模拟大鼠隐睾时的睾丸温度变化情况,同时对大鼠的伤害较小,操作比较简单。

The Exploration of a New Method in Rat Cryptorchidism Model Making

Objective In order to improve the method of setting up the model of rat cryptorchidism mode,we explored a new method and also examined its stability.Method Twenty-eight male Wistar rats were randomly divided into control（Ctrl） and model（Modl） group.Each rat was kept in a single cage.The Ctrl group can move freely during the experiment.The Modl group was tail-suspended for 3 weeks,and then reloaded for 8 weeks.Rats were sacrificed at the end of 3rd week and 11th week.The testis and epididymis were removed,weighted,then stained by Hematoxylin and Eosin Stainin,and examined under light microscopy.Results After 3-week tail-suspending,all rats’ testis slipped into enterocoelia.Compared with Ctrl Group,the weight of testis and epididymis were significantly decreased（P〈0.01）.After 8 weeks recovery,the weight of testis and epididymis did not increase to normal（P〈0.01）.With morphological examination,we found that after 3 weeks tail-suspending,seminiferous tubules were completely disorder and the spermatogonium were disappeared in the cauda epididymidis.The pathological changes were not improved（P〈0.01）at the end of 11th week.Conclusion 3-week tail-suspending can simulate the pathological changes of cryptorchidism syndrome.It is much easier to be done than the traditional one.Moreover,this method has less injury on the rat.Therefore,it can be used to set up a new model of cryptorchidism syndrome.