间充质干细胞-透明质酸-多聚赖氨酸治疗脊髓损伤的实验研究

目的研究间充质干细胞—透明质酸—多聚赖氨酸复合物治疗脊髓损伤的可行性,评价其治疗效果并探讨其可能机制。方法从人骨髓中分离、培养人骨髓间充质干细胞（human bone marrow mesenchymal stem cell,hBMSC）;制作大鼠脊髓半横断模型,按照实验分组分别将hBMSC、透明质酸-多聚赖氨酸（hyaluronic acid-poly-L-lysine,HA-PLL）、hBMSC-HA-PLL复合物注入损伤区域,单纯损伤组作为对照。术后按照不同时间点评价损伤和移植后的大鼠运动功能。8周后杀死大鼠,观察不同移植组体内轴突和血管生长的情况,对不同细胞、材料及复合物移植对大鼠脊髓损伤修复效果进行评估。结果 hBMSC移植组和hBMSC-HA-PLL移植组的大鼠运动功能的改善显著好于单纯损伤及HA-PLL移植组。电镜结果证实复合物移植组可显著促进轴突和血管生长,新生的轴突和血管结构较为完整。结论 hBMSC具有促进神经功能恢复的作用,将其与HA-PLL相结合,可以促进大鼠脊髓损伤修复,其机制可能包括材料框架作用和hBMSC在体内对大鼠神经细胞的营养作用以及促进微血管的生成。

Experimental Research of hBMSC-HA-PLL to Treat Spinal Cord Injury

Objective To investigate the feasibility of bone marrow mesenchymal stem cell（hBMSC） transplantation combined with hyaluronic acid poly-l-lysine（HA-PLL） to treat spinal cord injury.Methods hBMSC were extracted from human bone marrow and were cultured and expanded in vitro.The spinal cord of rat was hemi-transected,hBMSC,HA-PLL or hBMSC plus HA-PLL were transplanted alternatively after the injury.The motor function was assayed at different time points.Eight weeks later rats were sacrificed,the growth of axons and micro-blood vessels of different groups were checked,the therapeutic effect of cells,materials and the combination were assayed.Results The progression of rat locomotor function examined by BBB score showed that both groups of hBMSC transplant and hBMSC combined with HA-PLL have better recovery than hemi-transection group and HA-PLL group.Electron microscopy showed combination of hBMSC and HA-PLL can promote axonal and vascular growth.Conclusions hBMSC can promote neurological function recovery.Combination of stem cells and HA-PLL may get better effect.Based on these results,we deduced that the mechanism included the framework supporting effect of materials,the neurotrophic and angiogenesis effect of hBMSC.