大鼠爬板实验装置的设计制作及其在脑损伤实验中的应用

目的设计制作一种大鼠爬板实验装置,以使科研工作者能轻松快捷地通过爬板实验检测大鼠脑损伤后神经功能的改变,并提高实验数据的准确性。方法将成年雄性SD大鼠随机分为Tβ4组、PBS组和sham组。分别采用新装置和原装置对3组大鼠进行爬板实验,检测记录术后1～7d的神经功能缺失评分,并进行统计分析。结果用新装置进行检测：Tβ4组和PBS组的神经功能缺失评分均在逐步平缓下降,5 d后,仍可检测到神经功能缺失;两组之间的差异（P〈0.05）在3 d后,均可检出。Tβ4组与sham组之间的差异（P〈0.05）,前3d均可检出。PBS组与sham组之间的差异（P〈0.05）,术后一直可以检出。用原装置进行检测：Tβ4组和PBS组的神经功能缺失评分呈跳跃式下降,5 d后,不再能检出神经功能缺失;两组之间的差异（P〈0.05）,只在第4天时检出。Tβ4组与sham组之间的差异（P〈0.05）,只在前2 d检出。PBS组与sham组之间的差异（P〈0.05）,只前在前4 d检出。结论新的实验装置制作简单,实验操作便利;可以提高实验数据的准确性,能更好地检测出因实验因素所造成的统计学差异。

The Design and Making of an Experimental Apparatus for Rat Climbing Board Test and Application in the Rats with Cerebral Injury

Objective To design and make an experimental apparatus so that the researchers can easily and quickly detect the neural function deficits of rats after cerebral injury by means of climbing board test,and to improve the precision of the experimental data.Methods The male adult SD rats were randomly divided into three groups： Tβ4 group,PBS group and Sham group.From the 1st to the 7th day after operation,the new apparatus and the primary one were applied separately in the climbing board test to detect the neural function deficits of rats of the three groups.The nerve function loss score was recorded and analyzed by statistical methods.Results Results based on the new apparatus： Both the nerve function loss score of the Tβ4 group and that of the PBS group decreased gradually,and the neural function deficits could still be detected five days later.The statistical difference（P0.05）between the two groups could be checked out three days later.The statistical difference（P0.05）between the Tβ4 group and the Sham group could be detected in the earlier three days.The statistical difference（P 0.05）between the PBS group and the Sham group could be found during the seven days after operation.Results based on the primary apparatus： Both the nerve function loss score of the Tβ4 group and that of the PBS group decreased sharply,and the neural function deficits couldn＇t be detected five days later.The statistical difference between the two groups could be found only at the 4th day.The statistical difference（P0.05）between the Tβ4 group and the Sham group could be detected only in the earlier two days.The statistical difference（P0.05）between the PBS group and the Sham group could be found only in the earlier four days.Conclusion The new experimental apparatus is easily to be made and can be operated conveniently in experiment.It can increase the precision of experimental data,and has better function to detect the statistical differences resulted from the experimental factors.