应用手持激光器建立光化学法脑梗死动物模型

目的应用手持激光器作为光源建立光化学法局灶性脑梗死动物模型。方法将36只SD大鼠随机分为4组，即A组：开骨窗至硬脑膜，不注射玫瑰红，手持激光器照射5rain；B组：开骨窗至硬脑膜，注射玫瑰红，手持激光器照射5min；C组：保留颅骨内板，注射玫瑰红，手持激光器照射5min；D组：开骨窗至硬脑膜，注射玫瑰红，冷光源照射40rain。于术后24、48h对各组大鼠进行神经功能的行为学评分，进行MR扫描，术后48h处死动物，TTC染色测量梗死体积，光镜下观察病理改变，比较各组的模型制作成功率。结果不同方法进行动物模型制作时，24h神经功能的行为学评分有显著性差异，48h后差异消失。头部MR扫描显示，A组未见脑梗死形成，B组、c组全部有脑梗死灶形成，D组仅部分大鼠形成梗死灶，但体积明显较B、C组小，另外C组有2例合并硬膜外血肿。TTC染色A组未见梗死灶形成，B组、C组可见恒定的梗死灶，D组仅部分形成梗死灶。B、C、D组模型制作成功率分别为100％、80％、50％，将B组、C组合并为手持激光器组，与冷光源组（D组）比较，两种方法间有显著性差异（P=0．026）。结论应用手持激光器作为照射光源与冷光源相比，有更高的模型制作成功率。

Photochemical Ischemic Stroke Model Made by Hand-held Laser in Rats

Objective The objective of this study is to observe the effect of photochemical ischemic stroke model made by hand-held laser in rats. Methods 36 rats were randomly divided into four groups： group A, a skull hole was made deep to dura, irradiated by hand-held laser without rose-bengal injection for 5 minutes; groupB, a skull hole was made deep to dura, irradiated by hand-held laser for 5 minutes with rose-bengal injection ; group C, a skull hole was made deep to inner plate, irradiated by hand-held laser for 5 minutes with rose-bengal injection; group D, a skull hole was made deep to dura, irradiated by cold light source for 40 minutes with rose-bengal injection. Neurological deficit was evaluated 24 and 48 hours after surgery. MR scan was performed at 24 hours and the rats were killed at 48 hours, then the infarct volume was measured by TTC staining and the pathological changes was observed by light microscopy, comparing the success rate between two different irradiation sources. Results The neurological deficit differed at 24 hours but converged at 48 hours. MR scan showed no infarction in group A and some in group D, obvious infarction in all rats from group B and C, but epidural hematoma occured in two rats of group C. TTC staining showed no infarction in group A, an infarctionlesion occurred constantly in the left irradiated region in group B and C, but infarction ocurred only in half of the rats in group I）. The successful rate of model-made was 100% ,$0% and 50% in group B, group C and group D respectively. Combining group B and group C into hand-held laser group, compared with the cold light source group （group D） , there was a significant difference between two groups （P = 0. 026）. Conclusions In the procedure of photochemical ischemic stroke model made, compared with cold light source, the successful rate was higher when using hand-held laser as the irradiating source.