| 近交系剑尾鱼的STR遗传检测技术 |
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| 引用本文： | 魏杰,王洪,马丽颖,刘双环,岳秉飞.近交系剑尾鱼的STR遗传检测技术[J].中国实验动物学杂志,2009(12):66-69. |
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| 作者姓名： | 魏杰 王洪 马丽颖 刘双环 岳秉飞 |
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| 作者单位： | 中国药品生物制品检定所,北京100050 |
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| 基金项目： | 北京科技计划项目（D07080200720702）. |
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| 摘 要： | 目的建立近交系实验用鱼DNA检测方法，用于遗传质量控制。方法以RR-B、RW-H、BY-F三个近交系剑尾鱼的基因组DNA为主要研究对象，并以非选育剑尾鱼作对照，采用STR（short tandem repeat）引物扩增方法检测不同品系在DNA上的异同。设计10对STR引物，优化PCR电泳条件，使用相同的实验条件进行重复，以得到可靠的扩增条带，筛选出特异性引物。结果有4对引物可扩增出稳定条带，1对引物（AY204223）的PCR扩增条带在非选育群中表现多态性，在RR-B与BY-F系表型一致，与RW-H表型不同。这些STR位点能够区分剑尾鱼三个近交系品系。结论STR检测技术可望用于近交系剑尾鱼的遗传质量监测。
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| 关 键 词： | STR 剑尾鱼 近交系 遗传学技术 |
Development of a STR Method for Genetic Monitoring of Three Inbred Strains of Xiphophorus helleri |
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| WEI Jie,WANG Hong,MA Li-ying,LIU Shuang-huan,YUE Bing-fei.Development of a STR Method for Genetic Monitoring of Three Inbred Strains of Xiphophorus helleri[J].Chinese Journal of Laboratory Animal Science,2009(12):66-69. |
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| Authors: | WEI Jie WANG Hong MA Li-ying LIU Shuang-huan YUE Bing-fei |
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| Institution: | (National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China) |
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| Abstract: | Objective To develop a new STR （short tandem repeat） method for genetic monitoring of Xiphophorus helleri of three inbred strains. Method Ten STR primers were used to amplify the DNA of the inbred Xiphophorus strains RR-B, RW- H and BY-F. Results Four STR primers can produce stable bands. The amplified porducts of AY204223 exhibited polymorphism in wild swordtails, and displayed different band patterns among the three inbred Xiphophorus helleri. Conclusion The results indicate that the STR method could be used in genetic monitoring of special inbred Xiphophorus helleri. |
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| Keywords: | Short tandem repeat STR Xiphophorus helleri Inbred Strain Genetic monitoring techniques |
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