心脏特异表达NOL3转基因小鼠的建立

目的建立心脏特异表达NOL3转基因小鼠，用于研究该基因在心肌病发病中的作用。方法Western blot检测小鼠NOL3表达谱。构建aMHC-NOL3表达载体，显微注射法建立NOL3转基因小鼠。PCR鉴定转基因鼠的基因型，心脏超声检测转基因及野生型小鼠心脏功能及几何构型。结果NOL3在1月龄野生型鼠心脏、脑、骨骼肌中的高表达，在心脏中的表达不随年龄而改变。通过转基因小鼠的筛选，得到了3个NOL3转基因品系，其中1个品系心脏NOL3蛋白表达量与野生型鼠相比明显增加。单转NOL3基因的小鼠心脏功能及几何构型与野生型小鼠相比无显著变化。结论成功建立了心脏特异表达NOL3转基因小鼠，为进一步和心肌病小鼠模型杂交，研究该基因在心肌病发病中的作用提供了工具。

The Establishment of Heart Specific NOL3 Gene Transgenic Mice

Objective To establish nucleolar protein 3 （NOL3） transgenic mice and investigate its effect on the development of cardiomyopathy. Methods The NOL3 expression in the different tissues and different ages of mice was detected with western blot. fransgenic mice were generated by the method of microinjeetion. The genotype of transgenic lines was identified by PCR. The cardiac function and geometry were detected by echoeardiography. Results The western blot analysis showed that NOL3 was expressed in quite higher levels in the tissues of heart, muscle and brain than in other tissues. The NOL3 expression in the heart maintained a high level from newborn to adult. Three founders of heart specific α-MHC-NOL3 transgenic mice were established and one high-level expression line was identified. The transgenic gene itself did not affect the cardiac function and geometry compared with the wild type mice. Conclusion The transgenic mice with cardiac-specific expression of the human NOL3 gene were established successfully and it can be used to cross with the DCM and HCM model to investigate the function of NOL3 genc on the development of cardiomyopathy.