浙江省热原检查用兔敏感性状况调查

目的调查浙江省热原检查用兔对细菌内毒素的敏感性情况,为提高热原检查结果的准确性和可靠性提供参考。方法对全省所有取得生产许可证单位的家兔,用国家颁发的细菌内毒素标准品,＂热原检查法＂进行检查,剂量分别为5EU/Kg和10EU/Kg,记录并比较各单位家兔的平均升温值和升温率。结果对细菌内毒素,各兔场家兔的敏感性有一定的差异。静脉注射5EU/kg,平均升温值为0.40℃～0.87℃,升温率为35%～83%;静脉注射10EU/kg,平均升温值为0.74℃～1.16℃,升温率为70%～94%。结论不同生产单位的家兔对细菌内毒素的敏感性不同,有必要对热原检查用家兔进行细菌内毒素敏感性检查。

Investigation of the Sensitivity of the Rabbits for Testing Pyrogen in Zhejiang Province

Objective To investigate the sensitivity to bacterial endotoxin of the rabbits for testing pyrogen （RTP） in Zhejiang province. Methods The rabbits from all the farms that obtained production license in Zhejiang province were administrated with two doses of endotoxin （5 EU /kg and 10 EU /kg）. The average value and percentage of temperature elevated of the rabbits from different farms were recorded and compared in the pyrogen test. Results There were some differences on the sensitivity of RTP to bacterial endotoxin form different farms. When the rabbits were injected the endotoxin intravenously of 5 EU /kg or 10 EU /kg respectively,the average value of temperature were 0. 40℃ ～ 0. 87℃ or 0. 74℃ ～ 1. 16℃ and the percentage of temperature elevated were 35% ～ 83% and 70% ～ 94%. Conclusion Different rabbits from different farms show different sensitivity to bacterial endotoxin. It is necessary to test the sensitivity of RTP to bacterial endotoxin.