| 人源化小鼠人源细胞检测方法 |
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| 引用本文： | 陈炜,冯娟,施海霞,梁虹,张连峰.人源化小鼠人源细胞检测方法[J].中国实验动物学杂志,2010(7):63-66. |
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| 作者姓名： | 陈炜 冯娟 施海霞 梁虹 张连峰 |
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| 作者单位： | 中国医学科学院实验动物研究所北京协和医学院比较医学中心卫生部人类疾病比较医学重点实验室,北京100021 |
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| 基金项目： | 国家科技重大专项“艾滋病和病毒性肝炎等重大传染病防治”（2009ZX10004-307） |
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| 摘 要： | 目的人源化小鼠在人类疾病与再生医学研究中具有广泛应用,为获得一种理想的人源化小鼠检测方法,研究人特异性线粒体序列扩增作为人源化动物模型中人类DNA检测方法的可行性。方法设计人线粒体DNA特异性引物,并用该引物对人脐血造血干细胞嵌合体小鼠进行了检测。结果在人源化动物模型的人类DNA检测中,这种人线粒体DNA的PCR检测方法能够达到理想的特异性与灵敏性,是一种理想的嵌合体小鼠检测方法。结论获得了一种理想的人源化小鼠人源细胞检测方法。
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| 关 键 词： | 人源化小鼠 脐血干细胞 线粒体DNA |
Detection Method for Humanized Cells in Human-mouse Chimeras |
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| CHEN Wei,FENG Juan,SHI Hai-xia,LIANG Hong,ZHANG Lian-Feng.Detection Method for Humanized Cells in Human-mouse Chimeras[J].Chinese Journal of Laboratory Animal Science,2010(7):63-66. |
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| Authors: | CHEN Wei FENG Juan SHI Hai-xia LIANG Hong ZHANG Lian-Feng |
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| Institution: | (Key Laboratory of Human Disease Comparative Medicine,Ministry of Heath,Institute of Laboratory Animal Science,Chinese Academy of Medical Sciences ＆ Comparative Medical Center,Peking Union Medical College,Beijing 100021,China) |
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| Abstract: | Objective To extensively analyze the genotyping methodology for human-mouse chimeras,which is mandatory for research on regenerative medicine and some human diseases,a feasibility study was conducted to find out if amplification of human specific mitochondrial sequences could be applied to accurately and sensitively quantify of human genomic DNA in humanized animal model. Methods The primers of amplification of human specific mitochondrial sequences was designed and used to amplificate the humanized cells in human umbilical cord blood-derived cells-mouse chimeras. Results These results demonstrated that this new approach provided the necessary level of specificity and sensitivity. Conclusion This method comprises a simple and reliable assay system for studies of human-mouse chimeras. |
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| Keywords: | Human-mouse chimeras Human umbilical cord blood-derived cells Mitochondria DNA （mtDNA） |
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