云南猕猴mtDNA控制区全序列测定

目的测定云南猕猴线粒体DNA控制区全序列，对其进行鉴定及进化分析。方法利用PCR技术扩增猕猴线粒体DNA控制区全序列，结合GenBank中下载的猕猴参考序列（AY612638），采用多个生物学软件对序列碱基组成、同源性、转换／颠换比等遗传信息进行分析，并基于邻接法（NJ）和最小进化法（ME）构建系统进化树。结果云南猕猴线粒体DNA控制区全长为（1084-1089）bp，A、T、G和c四种碱基平均含量分别为29．9％、26．9％、12．3％和30．9％，A＋T含量（56．8％）高于G＋C含量（43．2％）。所分析序列间的同源性为91．5％-99．5％，平均核苷酸变异率为4．5％，变异类型包括转换、颠换、插入和缺失4种形式，转换／颠换比值平均为26．1。进化树显示云南猕猴存在两个平行进化的姐妹分支。结论本研究获得了云南猕猴mtDNA控制区全序列，为猕猴进化关系研究及mtDNA控制区功能研究奠定基础。

Determination of the Complete Region in Macaca mulatta Sequence of mtDNA Control from Yunnan Province

Objective To determine the complete sequences of the mitochondrial DNA control region in Macaca mulatta from Yunnan province. The complete sequences were analyzed for phylogenetic tree and nucleotide composition. Methods The complete sequences was amplified by polymerase chain reaction （PCR）. The nucleotide composition was analyzed by comparison with the known control region sequence （AY612638） downloaded from GenBank database. The homology, the rate of transition/transversion and phylogenetic tree constructed by neighbor-joining （NJ） and minimumevolution （ME） methods were also analyzed. Results The length of the mitochondrial DNA control region complete sequences were 1084 to 1089 bp,the average contents of A, T, G and C were 29.9% , 26.9% , 12. 3% and 30. 9% ,respectively. The content ofA ＋ T （56. 8% ） was higher than G ＋ C （43.2%） in the complete sequence. The homology of these sequences was 91.5%-99. 5%. The nueleotide mutational rate was 4. 5% on average, including transition, transversion, insertion and loss. The rate of transition/transversion was 26.1 on average. The phylogenetic analysis based on mitochondrial DNA control region showed that the Macaca mulatta of Yunan was divided into two sister groups geographically. Conclusions The complete sequences of the mitochondrial DNA control region of Macaca rnulatta has been determined. This study may be of importance for studies on evolution and function of the mitochondrial DNA control region of Macaca mulatta.