五种国产与进口小鼠病毒ELISA抗体检测试剂盒的比较研究

目的评价国产小鼠病毒抗体ELISA检测试剂盒。方法选择国产与进口小鼠淋巴细胞脉络丛脑膜炎病毒（LCMV）、肝炎病毒（MHV）、仙台病毒（SV）、腺病毒（MAV）、细小病毒（MPV）ELISA抗体检测试剂盒,进行敏感性、特异性、精密性、稳定性、可信度试验比较。结果国产与进口试剂盒：同种试剂盒之间灵敏度相差最低为2倍,差异显著（P〈0.05）,最高为16倍,差异极显著（P〈0.01）;特异性试验显示每种试剂盒,与其他4种病毒均无交叉反应;精密性试验显示5种试剂盒批内平均变异系数均小于10%;稳定性试验显示5种试剂盒相对偏差均小于25%;分别选择已知36份小鼠血清进行检测,国产和进口LCMV、MHV、SV、MPV符合率均为100%;国产MAV符合率为86.1%,进口MAV符合率均为100%,二者之间差异极显著（P〈0.01）。结论除国产MAV试剂盒敏感性、可信度低于进口外,国产LCMV、MHV、SV、MPV试剂盒与进口同种试剂盒相比,在敏感性、特异性、精密性、稳定性和可信度方面均良好。

Comparison of Five Domestic and Imported ELISA Kits for Detection of Mouse Virus Antibodies

Objective To evaluate the domestic ELISA kit for detection of mouse virus antibodies.Methods Domestic and imported ELISA kits were choosen to detect five mouse virus antibodies,including lymphocytic choriomeningitis virus（LCMV）,hepatitis virus（MHV）,Sendai virus（SV）,adenovirus（MAV）,and parvovirus（MPV）.The sensitivity,specificity,precision,stability,and reliability of the five kits were compared.Result There were significant differences in sensitivity of the same type domestic and imported kits,varying from at least 2 times（P0.05） up to 16 times（P0.01）,and there were no cross reaction with the other four types of viruses.Precision test showed that the batch average coefficient of variation of the five kits was less than 10%.Stability test showed that the relative deviation of the five kits was less than 25%.36 known mouse serum samples were tested with both the domestic and imported kits,respectively,and showed a coincidence rate of 100% for detection of LCMV,MHV,SV,and MPV.The domestic kit showed a coincidence rate of 86.1% in test for MAV,and that of the imported kit was 100%,with a very significant difference between them（P0.01）.Conclusions Except that the domestic MAV kit shows a lower sensitivity and credibility than that obtained with the imported kits,the domestic and imported LCMV,MHV,SV,MPV kits showed similar sensitivity,specificity,precision,stability and reliability.