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绿色荧光裸鼠血液生理生化指标检测分析
引用本文:尤金炜,张立波,方天,田小芸,刘彪,周森妹,梁磊,董敏,胡文娟,赵志刚,恽时锋.绿色荧光裸鼠血液生理生化指标检测分析[J].中国实验动物学杂志,2013(9):23-26.
作者姓名:尤金炜  张立波  方天  田小芸  刘彪  周森妹  梁磊  董敏  胡文娟  赵志刚  恽时锋
作者单位:南京军区南京总医院比较医学科,全军实验动物科普与伦理教育基地,全国科普教育基地,南京210002
基金项目:南京军区南京总医院院管课题(2012052).
摘    要:目的检测绿色荧光蛋白(green fluorescenc eprotein,GFP)转基因裸鼠血液生理生化指标,为将来的研究提供基础参考值。方法实验选用6~8周GFP转基因裸鼠及对照组BABL/C裸鼠雌雄各30只,取血测定血生化和血常规指标。结果①GFP转基因裸鼠与对照组BABL/C裸鼠比较,白细胞总数(WBC)、尿素(URE)、平均血红蛋浓度(MCHC)、葡萄糖(GLU)差异极显著(P〈0.01);血红蛋白(HGB)、红细胞分布宽度(RDW)、血小板计数(PLT)、尿酸(uA)差异显著(P〈0.05),其它指标差异不显著。②GFP转基因裸鼠雌雄间比较,红细胞分布宽度(RDW)、平均血红蛋白含量(MCH)、平均血红蛋浓度(MCHC)、血小板计数(PLT)、球蛋白(G)、胆固醇(TC)、HDL-胆固醇(HDL—TC)差异极显著(P〈0.01),白蛋白(ALB)、葡萄糖(GLU)差异显著(P〈0.05),其它指标差异不显著。结论转基因GFP转基因裸鼠的生理生化指标值在雄雌间有一定的差异,为相关的生物医学研究提供了基础数据。

关 键 词:GFP转基因裸鼠  血常规  血生化

Measurement of the blood biochemical parameters in GFP transgenic nude mice
YOU Jin-wei,ZHANG Li-bo,FANG Tian,TIAN Xiao-yun,LIU Biao,ZHOU Sen-mei,LIANG Lei,DONG Min,HU Wen-juan,ZHAO Zhi-gang,YUN Shi-feng.Measurement of the blood biochemical parameters in GFP transgenic nude mice[J].Chinese Journal of Laboratory Animal Science,2013(9):23-26.
Authors:YOU Jin-wei  ZHANG Li-bo  FANG Tian  TIAN Xiao-yun  LIU Biao  ZHOU Sen-mei  LIANG Lei  DONG Min  HU Wen-juan  ZHAO Zhi-gang  YUN Shi-feng
Institution:( Department of Comparative Medicine, Nanjing General Hospital of Nanjing Military Command, Army Education Base of Science and Ethics of Experimental Animal, National Science Education Base, Nanjing, 210002, China)
Abstract:Objective To measurement normal range of the blood routine values and blood biochemical parameters of the GFP Transgenic Nude Mice. To provide a basis for future research. Methods 6 8 weeks male and female 30 mice in each, were used in this experiment. Blood samples were measured using an automatic biochemical analyzer. Comparing the differences between GFP Transgenic Nude Mice with BALB/c Nude Mic eand sexes. Results (~)The comparison between GFP Transgenic Nude Mice and BALB/c Nude Mice revealed the values of WBC,URE,MCHC,GLU showed very significant difference ( P 〈 0. O1 ) ; HGB,RDW,PLT,UA difference ( P 〈 0. 05 ). (~)Making a comparison of the male and female of GFP Transgenic Nude Mice, the values of RDW,MCH,MCHC,PLT,G,TC,HDL-TC showed very significant difference ( P 〈 0. 01 ) ; ALB, GLU showed difference ( P 〈 0.05 ). Conclusion There were some differences to some blood indicators between the male and female GFP Transgenic Nude Mice, the above data could be useful in the biomedical researches.
Keywords:GFP transgenic nude mice  Blood routine  Blood biochemical parameters
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