| 造血干细胞全标记红色和绿色荧光转基因小鼠的筛选 |
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| 引用本文： | 张晓娟,李小颖,施海霞,葛文平,梁虹,张连峰.造血干细胞全标记红色和绿色荧光转基因小鼠的筛选[J].中国实验动物学杂志,2011(5):14-17,82. |
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| 作者姓名： | 张晓娟 李小颖 施海霞 葛文平 梁虹 张连峰 |
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| 作者单位： | 中国医学科学院,北京协和医学院,医学实验动物研究所,卫生部人类疾病比较医学重点实验室,国家中医药管理局人类疾病动物模型三级实验室,北京100021 |
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| 基金项目： | 国家科技重大专项“艾滋病和病毒性肝炎等重大传染病防治”（2009ZX10004-307）; 十一五新药专项支持（2009ZX09501-026） |
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| 摘 要： | 目的对五种荧光转基因小鼠造血干细胞中的荧光标记细胞进行分析,筛选造血干细胞全标记红色和绿色荧光转基因小鼠,为造血干细胞分化机制体内示踪研究提供理想的动物模型。方法采用活体荧光影像系统对两种红色荧光转基因小鼠品系C57BL/6J-TgN（CAG-DsRed-1和CAG-DsRed-2）ZLFILAS和三种绿色荧光转基因小鼠品系C57BL/6J-TgN（CAG-EGFP-1、CAG-EGFP-2和CAG-EGFP-3）ZLFILAS的荧光标记进行比较;采用流式细胞术检测各转基因小鼠的骨髓lin（-）c-kit（＋）Sca-1＋（LSK）造血干细胞荧光标记细胞比率,根据标记比率筛选造血干细胞全标记红色和绿色荧光转基因小鼠。结果活体荧光影像分析表明转基因小鼠均系统性表达红色或绿色荧光。流式细胞术检测表明LSK造血干细胞中高度表达红色和绿色荧光,其中,C57BL/6J-TgN（CAG-DsRed-1）ZLFILAS和C57BL/6J-TgN（CAG-EGFP-1）ZLFILAS的造血干细胞全部为荧光标记细胞。结论筛选获得在造血干细胞中全标记的红色和绿色荧光转基因小鼠,可为造血干细胞体内研究提供有效示踪工具。
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| 关 键 词： | 红色荧光蛋白 绿色荧光蛋白 转基因 造血干细胞 小鼠 |
Selection of Transgenic Mouse Models with Totally Labeled Fluorescence in Hematopoietic Stem Cell |
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| ZHANG Xiao-juan,LI Xiao-ying,SHI Hai-xia,GE Wen-ping,LIANG Hong,ZHANG Lian-feng.Selection of Transgenic Mouse Models with Totally Labeled Fluorescence in Hematopoietic Stem Cell[J].Chinese Journal of Laboratory Animal Science,2011(5):14-17,82. |
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| Authors: | ZHANG Xiao-juan LI Xiao-ying SHI Hai-xia GE Wen-ping LIANG Hong ZHANG Lian-feng |
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| Institution: | (Key Laboratory of Human Disease Comparative Medicine,Ministry of Heath;Institute of Medical Laboratory Animal Science,Chinese Academy of Medical Sciences;Key Laboratory of Human Diseases Animal Models,State Administration of Traditional Chinese Medicine;Beijing Union Medicine College,Beijing 100021,China) |
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| Abstract: | Objective To compared the ratios of fluorescence labeling cells to the lin^（-）c-kit^（＋）Sca-1^＋（LSK） stem cells from the five transgenic mouse lines,selected the mouse lines of totally fluorescence labeled,for tracking the hematopoietic stem cells and in vivo fluorescence imaging analysis.Methods The expressions of the fluorescent protein in vivo were observed under in vivo fluorescence imaging system,the expression of those genes in the stem cells were investigated with flow cytometry.Results The fluorescent protein was expressed in most tissues in the five transgenic mice.The fluorescent protein expressions were observed in almost all LSK stem cells of C57BL /6J-TgN（CAG-DsRed-1） ZLFILAS and C57BL /6J-TgN（CAG-EGFP-1） ZLFILAS.Conclusions Two transgenic mouse lines were indicated in which LSK stem cells were marked completely by red or green fluorescence protein separately,suggesting the two mouse lines may be useful tools for in vivo tracking the hematopoietic stem cells. |
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| Keywords: | Red fluorescent protein Green fluorescent protein Transgene models Hematopoietic stem cell Mouse |
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