无创遥测技术观察运输应激对Beagle犬部分生理指标的影响

目的应用无创遥测技术观察运输应激对Beagle犬部分生理指标的影响。方法 16只Beagle犬随机分成两组（每组8只）,即对照组和运输应激组,并利用大动物无创生理信号遥测技术,分别监测清醒自由活动状态下对照组和运输应激组应激4 h后Beagle犬的心电图、活动、皮肤温度和呼吸参数的变化。结果 Beagle犬心率、RR间期、QT间期、活动、皮肤温度、呼吸均具有明显的昼夜节律变化（P〈0.01）;与对照组比,运输应激后Beagle犬心率、活动度、呼吸频率、每分钟通气量和潮气量均显著增加（P〈0.01）,RR间期、PR间期、皮肤温度均显著降低（P〈0.01）,相关分析表明运输应激对Beagle犬心率、活动、皮肤温度和呼吸频率具有显著的相关性（P〈0.05,P〈0.01）。结论运输应激可引起Beagle犬生理学指标的明显改变,但除皮肤温度外,运输应激（4 h）对Beagle犬昼夜节律变化破坏不明显;利用无创遥测技术平台可建立理想的Beagle犬生物模型,可用于动物福利和药理毒理学评价研究。

Effect of Transportation Stress on Physiological Parameters in Beagle Dogs Based on Noninvasive Telemetry Technology

Objective To study the possible influence of transportation stress on some physiological parameters in Beagle dogs based on noninvasive telemetry measurement.Methods Sixteen Beagle dogs were randomly divided into two groups（n = 8 in each）： control group and transportation stress group.24 h consecutive monitoring electrocardiogram（ECG）,activity,skin temperature and respiratory parameters were recorded in the control group and after transportation stress for 4 h in transportation group,from conscious and unrestrained beagle dogs using a noninvasive telemetry system.Results Heart rate,RR interval,QT interval,activity,skin temperature and respiration had significant circadian rhythm changes（P 0.01）.Compared with the control group,heart rate,activity,respiratory frequency,minute volume and tidal volume were significantly increased in the transportation stress group（P 0.01）,while RR interval,PR interval and skin temperature were significantly decreased（P 0.01）.Correlation analysis showed that there was remarkable correlation between transportation stress and heart rate,activity,skin temperature and respiratory frequency（P 0.05,P 0.01）.Conclusions Transportation stress for 4 h may cause significant changes of physiological parameters in Beagle dogs,but except for skin temperature,it does not impair the circadian rhythm change in Beagle dogs.Application of noninvasive telemetry technology can be useed to establish an ideal living model of Beagle dogs,and it is helpful in researches of animal welfare and pharmacology and toxicology.