非酒精性脂肪性肝病兔肝组织硫化氢与一氧化氮的关系

目的观察非酒精性脂肪性肝病（NAFLD）兔肝组织硫化氢（H2S）、一氧化氮（NO）浓度,探讨H2S、NO在NAFLD发病中的作用。方法 40只日本大耳白兔数字法随机分为重度NAFLD组（重度组）、轻度NAFLD组（轻度组）、空白对照组（对照组）。重度组给予高脂饲料160 g/（兔.d）,轻度组给予高脂饲料80 g/（兔.d）＋普通饲料80 g/（兔.d）,对照组给予普通饲料160 g/（兔.d）。均饲养13周。实验前后采集血浆标本,同步检测甘油三酯（TG）、胆固醇（TC）;肝组织匀浆检测NO、H2S浓度。肝组织HE染色,光镜观察肝脏病理学。结果⑴TC、TG：饲养前重度组、轻度组、对照组TC、TG比较差异无统计学意义（P〉0.05）,饲养后重度组TC、TG分别为（32.12±1.25）、（6.02±2.12）mmol/L,轻度组TC、TG分别为（18.34±2.10）、（4.39±1.93）mmol/L,均高于饲养前（P﹤0.01）,饲养后重度组TC、TG高于轻度组（P﹤0.01）。⑵肝组织NO：重度组（132.4±20.7μmol/g蛋白）和轻度组（95.4±19.8μmol/g蛋白）肝组织NO浓度显著高于对照组（74.9±34.7μmol/g蛋白,P﹤0.01）,重度组又显著高于轻度组（P﹤0.01）。⑶肝组织H2S浓度：与对照组比较,重度组和轻度组肝组织H2S明显下降（P﹤0.01）,重度组与轻度组比较下降更显著（P﹤0.05）。⑷肝脏病理学：重度组肝脏病理学改变呈重度NAFLD,轻度组呈轻度～中度NAFLD。结论 NO、H2S参与NAFLD的发生、发展,通过干预NO、H2S防治NAFLD可能是未来方向。

Relationship of Nitric Oxide and Hydrogen Sulfide in the Liver Tissue of Rabbit with Nonalcoholic Fatty Liver Disease

Objective To observe the changes of nitric oxide（NO） and hydrogen sulfide（H2S） levels in the liver tissue of rabbit with nonalcoholic fatty liver disease（NAFLD）,and explore the effect of NOand H2 S in the pathogenesis of NAFLD.Methods Forty Japanese white rabbits were randomly divided into severe NAFLD group,mild NAFLD group,and blank control group.The severe NAFLD group was given high fat diet 160 g /rabbit.day,the mild NAFLD group was given high fat diet 80 g /rabbit.day and normal diet 80 g /rabbit.day,and the blank control group was given normal diet160 g /rabbit.day.The raising cycle was 13 weeks.Plasma samples were collected before and after the experiment,and the concentration of triglyceride（TG）,cholesterol（TC）,and the levels of NO and H2 S in the liver tissuewere determined.Pathological changes of the liver tissue were examined by light microscopy with HEstaining.Results（1） TC and TG levels： There was no significant difference between the mild and blank control groups（P 0.05）.After the high diet feeding,the TC and TG were（32.12 ± 1.25） and（6.02 ± 2.12） nmol /L in the severe NAFLD group,（18.34 ± 2.10） and（4.39 ± 1.93）nmol/L in the mild NAFLD group,respectively,before experiment（P 0.01）,and those in the severe were also significantly higher than thosein the mild NAFLD group（P 0.01）.（2） The NO level in the liver tissue： It was（132.4 ± 20.7）μmol /g.pro in the severe,（95.4 ± 19.8）μmol /g.pro in the mild NAFLD group,and（74.9 ± 34.7） μmol/g.pro in the blank control group,with a very significant difference between the severe and mild NAFLD groups,and between the two NAFLD and the blank control groups.（3） The H2S level in the liver tissue of the severe and mild NAFLD groups was significantly decreased,compared with that in the blank control group（P 0.01）,and between the severe and mild NAFLD groups（P 0.05）.（4） Liver pathology： Moderate changes were seen in the severe NAFLD group,slight to moderate changes in the mild NAFLD group,and normal histological appearance in the liver tissue of the blank control group.Conclusions NO and H2S participate the pathogenesis of nonalcoholic fatty liver disease.It is possible that intervention of the NO and H2S may become another future direction in prevention of this disease.