| 鸡胚尿囊膜（HET-CAM）眼刺激替代试验对产品及原料评价的比较 |
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| 引用本文： | 邱璐,李小林,卞俐娜,山口能宏.鸡胚尿囊膜（HET-CAM）眼刺激替代试验对产品及原料评价的比较[J].中国实验动物学杂志,2011(3):27-30,35,81. |
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| 作者姓名： | 邱璐 李小林 卞俐娜 山口能宏 |
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| 作者单位： | [1]上海出入境检验检疫局,上海200135 [2]日本小林制药株式会社,大阪 |
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| 摘 要： | 目的比较鸡胚尿囊膜试验（HET-CAM）作为一种眼刺激替代方法对产品或原料的评价。方法采用HET-CAM和兔眼Draize试验方法,对19种原料和23种化妆品及家用洗涤产品眼刺激性进行检测,对体内体外试验的结果进行统计比较。结果比较体内体外试验,原料和产品的kappa系数分别为0.826,0.531;灵敏度分别为100%,81.8%;特异度分别为85.7%,77.8%。结论 HET-CAM可作为Draize试验的替代试验,HET-CAM系统更适合对单纯化学品原料进行眼刺激试验。
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| 关 键 词： | 鸡胚尿囊膜试验 Draize眼刺激试验 替代试验 |
Comparison of HET-CAM Eye Irritation Test for Products and Ingredients |
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| Institution: | QIU Lu,LI Xiao-lin,BIAN Li-na,YOSHIHIRO Yamaguchi.(1.Shanghai Entry-Exit Inspection and Quarantine Bureau of the P.R.C,Shanghai 200135,China;2.KOBAYASHI Pharmaceutical Co.,Ltd.,Osaka 567-0057,Japan) |
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| Abstract: | Objective To compare the adaptability of HET-CAM eye irritation test for products and ingredients.Methods Test 19 ingredients and 23 products by HET-CAM and Draize eye irritation protocol.Compare the data from in vitro and in vivo test.Result Compare in vitro data and in vivo data,The Kappa coefficient,sensitivity,specificity are 0.826,100%,85.75 for ingredient.0.531,81.8%,77.8% for cosmetics as well.Conclusion HET-CAM eye irritation test can be used to be alternative test of Draize test.HET-CAM system is more suitable for ingredients test. |
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| Keywords: | HET-CAM tset Draize eye irritation test Alternatives |
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