HIV-1 p24和SIV p27两种ELISA试剂盒检测SIV p27抗原的比较

目的由于检测SIV p27抗原试剂盒来源困难,有时不稳定,鉴于HIV-1 p24与SIVp27有较强的交叉抗原,本研究比较HIV-1 p24和SIV p27两种ELISA试剂盒检测SIV p27抗原得出的结果是否存在一定的相关性。方法 HIV-1 p24和SIV p27两种ELISA试剂盒定性和定量检测样品中SIV p27抗原,并对检测结果进行回归和相关分析。结果 HIV-1 p24和SIV p27两种ELISA试剂盒检测SIV p27抗原的灵敏度分别是150 pg/mL和62.5 pg/mL。两种试剂盒检测病毒液和血浆中SIV p27抗原的定性结果一致。定量结果的统计分析得出病毒液的直线回归决定系数R2=0.857,直线相关系数r=0.926,P〈0.01,直线正相关程度较高;血浆的直线回归决定系数R2=0.512,直线相关系数r=0.716,P〈0.05,直线正相关程度较低。结论 HIV-1 p24 ELISA试剂盒能够替代SIVp27 ELISA试剂盒定性检测病毒液和血浆中SIV p27抗原,但只能定量检测病毒液中SIV p27抗原。

Comparison of the Efficacy of HIV-1 p24 ELISA Kit and SIV p27 ELISA Kit in Detection of SIV p27 Antigen

Objective The ELISA kit for HIV-1 p24 is sometimes used for SIV p27 detection.The aim of this study was to eclarify whether there is any dependence and correlation between the results of detecting SIV p27 antigen by HIV-1 p24 ELISA kit and SIV p27 ELISA kit.Methods SIV p27 antigens were detected qualitatively and quantitatively by HIV-1 p24 ELISA kit and SIV p27 ELISA kit,respectively,and the results were compared by regression and correlation analysis.Results The sensitivity of detecting SIV p27 antigen was 150 pg/mL by HIV-1 p24 ELISA kit while 62.5 pg/mL by SIV p27 ELISA kit.The qualitative results obtained by HIV-1 p24 kit consisted with those by SIV p27 kit on detecting SIV p27 antigen in virus stock and plasma.The statistical analysis of quantitative results indicated linear regression R2 = 0.857,linear correlation r = 0.926,P 0.01 in virus stock,while R2 = 0.512,r = 0.716,P 0.05 in plasma.The quantitative results turned out to be positive linear regression,which was higher in virus stock and lower in plasma.Conclusions HIV-1 p24 ELISA kit can be used for detecting SIV p27 antigen in virus stock and plasma qualitatively replacing SIV p27 ELISA kit,but quantitatively was appropriate in virus stock only.