α-生育酚对脑皮层神经元线粒体的保护作用 |
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引用本文: | 梁伟伦,;黄慧玲,;武俏丽,;王辰,;张文治,;苏心,;只达石.α-生育酚对脑皮层神经元线粒体的保护作用[J].中国实验动物学杂志,2007(10):585-588. |
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作者姓名: | 梁伟伦 ;黄慧玲 ;武俏丽 ;王辰 ;张文治 ;苏心 ;只达石 |
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作者单位: | [1]天津医科大学,天津300072; [2]天津环湖医院,天津300071 |
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基金项目: | 天津市“211重中之重”学科建设转专项基金资助项目.项目编号:05YFGD5F02500. |
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摘 要: | 目的探讨α-生育酚对神经元线粒体的保护作用。方法将神经元细胞进行分组,分为:(1)正常对照组,(2)单纯氧自由基损伤组,(3)α-生育酚保护组。利用Fenton反应造成神经元细胞氧自由基损伤,用激光共聚焦显微镜观察各组神经元JC-1染色结果,并检测各组神经元琥珀酸脱氢酶(SDH)和细胞色素氧化酶(CCO)活性。结果(1)JC-1染色结果分析:α-生育酚保护组神经元线粒体功能强于单纯氧自由基损伤组。(2)SDH和CCO酶活性分析:α-生育酚保护组神经元SDH和CCO酶活性高于单纯氧自由基损伤组。结论α-生育酚可以有效对抗氧自由基对神经元线粒体的损伤。
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关 键 词: | α生育酚 神经元 线粒体 |
Protective Effect of α-Tocopherol on the Mitochondria of Cortex Neurons |
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Institution: | LIANG Wei-lun, HUANG Hui-ling, WU Qiao-li, WANG Chen, ZHANG Wen-zhi, SU Xin, ZHI Da-shi (1. Tianjin Medical University, Tianjin 300072, China; 2. Tianjin Huanhu Hospital, Tianjin 300071) |
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Abstract: | Objective To evaluate the protective effect of α-tocopherol on the mitochondria of cortex neurons. Methods Neurons were divided into 3 groups: (1) normal group, (2) group of oxidative damage, and (3) group under α-tocopherol protection. Oxidative damage was induced by Fenton reaction in groups (2) and (3). The neurons were stained with JC-1 and examined the changes of mitochondria under confocal laser scanning microscope. The activity of SDH and CCO in the extracellular fluid was determined. Results 1. Analysis of JC-1 staining: α-tocopherol reduced the oxidative free radical-induced damage in mitochondria of neurons. 2. Analysis of SDH and CCO activity: The activity of SDH and CCO of group (3) was significantly higher than that of group (2). Conclusion α-tocopherol can effectively protect the mitochondria of neurons from damage induced by oxidative free radicals. |
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Keywords: | α-tocopherol Neuron Mitochondria |
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