| 利用雌激素构建小鼠淋病奈瑟菌感染模型 |
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| 引用本文： | 蒋桂花,;李国才,;夏怡吉,;陈荣,;季明春.利用雌激素构建小鼠淋病奈瑟菌感染模型[J].中国实验动物学杂志,2007(1):5-7. |
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| 作者姓名： | 蒋桂花 ;李国才 ;夏怡吉 ;陈荣 ;季明春 |
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| 作者单位： | [1]扬州大学比较医学中心,扬州225009; [2]扬州大学医学院病原生物学与免疫学教研室,扬州225001 |
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| 基金项目： | 江苏省自然科学基金项目（BK2005406）;江苏省应用基础研究基金项目（BJ95122）;扬州大学生命科学群基金项目（200308）. |
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| 摘 要： | 目的探讨用雌激素构建淋病奈瑟菌感染小鼠模型的方法。方法淋病奈瑟菌WHO-A接种经皮下注射苯甲酸雌二醇和真皮下埋植尼尔雌醇片（雌三醇）预处理的雌性ICR小鼠，小鼠阴道拭子接种T-M选择培养基培养分离淋病奈瑟菌，取小鼠阴道分泌物涂片染色观察淋病奈瑟菌感染情况，比较两种雌激素对小鼠淋病奈瑟菌感染的差异。结果与对照组相比，苯甲酸雌二醇小鼠体内淋病奈瑟菌有短时间的定植，而尼尔雌醇组与对照组无差异。结论雌激素（尤其是雌二醇）有利于淋病奈瑟菌在小鼠阴道内的定植。
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| 关 键 词： | 雌激素 淋病奈瑟菌 小鼠 模型 动物 |
Model Establishment of Neisseria gonorrhoeae Infection in Mouse by Using Estrogen |
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| Institution: | JIANG Gui-hua , LI Guo-cai , XIA Yi-ji , CHEN Rong , JI Ming-chun (1. The Comparative Medical Center of Yangzhou University ,Yangzhou 225009, China; 2. Department of Pathogeniology and Immunology, Medical college, Yangzhou University, Yangzhou 225001, China) |
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| Abstract: | Objective To find out a method of constructing a Neisseria gonorrhoeae genital infection mouse model. Method In our experiments, ICR mice were injected Estradiol （E2） and inplanted Estriol （E3） pellet respectively, then challenged with Neisseria gonorrhoeae WHO-A in the female mice vagina. The genital swab were verified by T-M culture media, the vaginal excretes smears were stained to determine different effect of the hormone on mouse Neisseria gonorrhoeae infection. Result Compared with non-estrogen-treated mice, estradiol（E2） treated mice have Neisseria gonorrhoeae short-lived in the vagina. And there was no gonococci in estriol （E3） treated mice like control group. Conclusion Estrogen, especially estradiol （E2） has the effect on promoting Neisseria gonorrhoeae vaginal colonization in mice. |
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| Keywords: | Estrogen Neisseria gonorrhoeae Mice Model animal |
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