| 小鼠腹膜透析模型的构建及腹膜转运关键蛋白的表达 |
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| 引用本文： | 张靖华,樊怡,马健飞,王力宁.小鼠腹膜透析模型的构建及腹膜转运关键蛋白的表达[J].中国实验动物学杂志,2008(4):18-20. |
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| 作者姓名： | 张靖华 樊怡 马健飞 王力宁 |
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| 作者单位： | 中国医科大学附属第一医院肾内科,沈阳110001 |
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| 摘 要： | 目的构建小鼠腹膜透析模型,以腹膜平衡试验（PET）曲线描述其转运特征,并观察腹膜转运关键蛋白在小鼠腹膜的表达。方法以C57BL/6小鼠为动物模型,通过PET建立透析模型,分别进行生化测定描绘PET曲线,以及蛋白印迹进行关键蛋白的检测。结果C57BL/6小鼠的PET曲线图形与人类接近。AQP-1、eNOS、Cav-1在小鼠腹膜均有稳定的表达。结论该模型结果可靠,代表性强,适合推广。
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| 关 键 词： | 模型 小鼠 腹膜平衡试验 关键蛋白 |
Establishment of a Mouse Model of Peritoneal Dialysis and the Expression of Key Transport-Related Protein on the Peritoneum |
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| ZHANG Jing-hua,FAN Yi,MA Jian-fei,Wang Li-ning.Establishment of a Mouse Model of Peritoneal Dialysis and the Expression of Key Transport-Related Protein on the Peritoneum[J].Chinese Journal of Laboratory Animal Science,2008(4):18-20. |
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| Authors: | ZHANG Jing-hua FAN Yi MA Jian-fei Wang Li-ning |
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| Institution: | (Department of Nephrology, The First Hospital of China Medical University, Shenyang 110001, China) |
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| Abstract: | Objective To establish a mouse model of peritoneal dialysis （PD）, describe the transport status of those mice by peritoneal equilibration test （PET） and Observe the expression of key transport-related protein on the peritoneum. Methods C57BL/6 mice were used as animal candidates. The PD model was set up by PET. A series of PET curves were obtained after biochemical measurement. The expression of key proteins was detected by Western blot. Resluts The PET curves of C57BL/6 mice are close to those of patients. AQP-1, eNOS and Cav-1 were all stably expressed on peritoneum. Conclusion The mouse model described in this study is relaible, with good representativeness, and deserves generalization. |
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| Keywords: | Mouse model Peritoneal dialysis Peritoneal equilibration test Key protein |
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