改良大鼠左心室插管术及心衰大鼠左心功能指标的测定

目的完善大鼠左心室插管技术并确定充血性心力衰竭（CHF）大鼠心功能指标参数。方法将80只成年雄性SD大鼠随机分为2组：假手术组（SH）,腹主动脉缩窄模型组（CAA）。采用腹主动脉部分缩窄法制作CHF大鼠模型,BL-420E＋生物信号采集系统测定心功能参数。观察比较2组大鼠第6周后测定的心功能的各项指标。结果（1）经进一步完善大鼠左心室插管技术,成功率明显提高。（2）CAA组大鼠心功能明显减低,左室重量指数（LVMI）、左室舒张末压（LVEDP）升高,左心室内压上升、下降的最大变化速率（±dp/dtmax）下降（P〈0.01）。结论改良大鼠左心室插管术提高成功率,心衰大鼠的心功能指标明显改变。

An Improved Method of Left Ventricular Cannulation and Measurement of Cardiac Function Parameters in Rats with Congestive Heart Failure

Objective To improve the skill of left ventricular cannulation and observe the changes of cardiac function parameters in rats with congestive heart failure （CHF）. Methods Eighty adult male SD rats were randomly divided into two groups：coarctation in the abdominal aorta （CAA） group and sham operated （SH） group. To apply the method of partial CAA to generate rat CHF model. The cardiac function parameters were measured by a BL-420E ＋ biosignal collection system and the measured parameters during 6 weeks were compared between the two groups. Results The success rate was apparently elevated up to 80% by improving the skill of left ventricular cannulation.The cardiac function in rats of CAA group was degraded. The left ventricular mass index （LVMI） and left ventricular end-diastolic pressure （LVEDP） were increased in rats of CAA group. However, the left ventricular pressure ± dp/dtmax was decreased in the same group （ P 〈 0.01 ）. Conclusion The success rate is increased by the improved skill of left ventricular cannulation, and the indexes of cardiac function are changed apparently.