松子壳多糖对小鼠主要免疫细胞功能的影响

目的探讨松子壳多糖（pine nut shell polysaccharide,PSP）对小鼠主要免疫细胞的影响。方法应用MTT法测定PSP对小鼠脾淋巴细胞的毒性和对ConA或LPS诱生小鼠脾T、B淋巴细胞的转化,用中性红吞噬试验测定腹腔巨噬细胞的吞噬功能,应用乳酸脱氢酶释放法测定NK细胞的杀伤活性。结果 PSP对脾细胞毒性很低,各种浓度对小鼠脾淋巴细胞的增殖均有较强的促进作用（P〈0.01）;PSP在浓度50～300μg/mL时,明显促进T淋巴细胞的转化（P〈0.01）,但是当浓度达到300μg/mL时表现出一定的抑制作用（P〉0.05）;PSP在25～200μg/mL时显著促进了小鼠脾B淋巴细胞的转化（P〈0.05）,但是当浓度达到300μg/mL时,抑制作用极显著（P〈0.01）;不同浓度均可以增强小鼠腹腔巨噬细胞吞噬中性红的能力及其代谢功能,当浓度在100μg/mL时能显著的促进巨噬细胞吞噬中性红的能力（P〈0.01）;PSP在浓度100～300μg/mL时,能极显著的促进NK细胞对Yac-1的杀伤作用（P〈0.01）,当浓度达到400μg/mL,对NK细胞杀伤性的促进作用开始减弱。结论 PSP对小鼠脾淋巴细胞的毒性较低,能增强免疫细胞活性,有望成为新一代免疫调节剂。

The Influence of Pine Nut Shell Polysaccharide on Immunological Function of Mouse

Objective To study the influence of pine nut shell polysaccharide （PSP） on mouse splenic lymphocytes. Methods Proliferation of T and B lymphocytes induced by ConA or LPS were determined by using MTT assay. Peritoneal macrophage function was determined by using neutral red approach. NK cell competence was determined by using lactate dehydrogenase approach. Results The results showed that PSP cell toxicity to splenic lymphocytes is very low,it remained higher immunologic competence on splenic lymphocytes even if the polysaccharide density achieves 1000 μg/mL. PSP of different concentrations can promote T and B Lymphocyte proliferation,and PSP significantly enhanced T lymphocyte proliferation induced by ConA ranging from 100 μg/mL to 300 μg/mL （P〈0. 01）. Different density PSP could enhance the function of peritoneal macrophage englobing neutral red and its metabolism. PSP could improve effect of NK cells against YAC-1. Conclusions PSP has the immunity activeness strongly,the toxic low characteristic. Thus,this polysaccharide seems to be a good candidate for the development of immunity conditioner.