Optimum conditions for the biological production of lactic acid by a newly isolated lactic acid bacterium,Lactobacillus sp. RKY2 |
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Authors: | Young-Jung Wee Jin-Nam Kim Jong-Sun Yun Hwa-Won Ryu |
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Institution: | (1) School of Biological Sciences and Technology, Chonnam National University, 500-757 Gwangju, Korea;(2) Department of Material Chemical and Biochemical Engineering, Chonnam National University, 500-757 Gwangju, Korea;(3) BioHelix, Biotechnology Industrialization Center, Naju, 520-811 Jeonnam, Korea |
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Abstract: | Lactic acid is a green chemical that can be used as a raw material for biodegradable polymer. To produce lactic acid through
microbial fermentation, we previously screened a novel lactic acid bacterium. In this work, we optimized lactic acid fermentation
using a newly isolated and homofermentative lactic acid bacterium. The optimum medium components were found to be glucose,
yeast extract, (NH4)2HPO4, and MnSO4. The optimum pH and temperature for a batch culture ofLactobacillus sp. RKY2 was found to be 6.0 and 36°C, respectively. Under the optimized culture conditions, the maximum lactic acid concentration
(153.9 g/L) was obtained from 200 g/L of glucose and 15 g/L of yeast extract, and maximum lactic acid productivity (6.21 gL−1h−1) was obtained from 100 g/L of glucose and 20 g/L of yeast extract. In all cases, the lactic acid yields were found to be
above 0.91 g/g. This article provides the optimized conditions for a batch culture ofLactobacillus sp. RKY2, which resulted in highest productivity of lactic acid. |
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Keywords: | batch fermentation culture conditions homofermentation lactic acid Lactobacillus |
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