Production of mycelial biomass and exo-polymer by <Emphasis Type="Italic">Hericium erinaceus</Emphasis> CZ-2: Optimization of nutrients levels using response surface methodology |
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| Authors: | Fengjie Cui Zhiqiang Liu Yin Li Lifeng Ping Liying Ping Zhicai Zhang Lin Lin Ying Dong Daming Huang |
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| Institution: | 1.School of Food and Biotechnology,Jiangsu University,Zhenjiang,China;2.Department of Food, Agricultural, and Biological Engineering,The Ohio State University,Wooster,USA;3.Institute of Bioengineering,Zhejiang University of Technology,Hangzhou,China;4.Department of Chemical and Biological Science, NSF-I/UCRC Center for Biocatalysis and Bioprocessing of Macromolecules,Polytechnic Institute of New York University,Brooklyn,USA;5.Department of Plant Science,North Dakota State University,Fargo,USA;6.Institute of Quality and Standard for Agricultural Products,Zhejiang Academy of Agricultural Sciences,Hangzhou,China |
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| Abstract: | The Doehlert experimental design was used to optimize the production of mycelial biomass and exopolymer from Hericium erinaceus CZ-2 in this study. Statistical analysis showed that the linear and quadric terms of 3 variables: corn flour, yeast extract,
and corn steep liquor had significant effects. The optimized combination of these 3 variables was confirmed through validation
experiments. The optimal conditions for higher production of mycelial biomass (19.92 g/L) were estimated when the media composition
concentrations were set as: 30.85 g/L, corn flour; 2.81 g/L, yeast extract; 16.9 mL/L, corn steep liquor; 10 g/L, glucose;
1 g/L, KH2PO4; and 0.5 g/L, MgSO4·7H2O; while a maximal exo-polymer yield (1.653 g/L) could be achieved when setting concentrations of: 32.71 g/L, corn flour;
2.35 g/L, Yeast extract; 14.42 mL/L, Corn steep liquor; 10 g/L, glucose; 1 g/L, KH2PO4; and 0.5 g/L, MgSO4·7H2O. The upscale production was also investigated using a 15 L fermentor using the optimized medium. |
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