Development of a serial bioreactor system for direct ethanol production from starch using<Emphasis Type="Italic">Aspergillus niger</Emphasis> and<Emphasis Type="Italic">Saccharomyces cerevisiae</Emphasis> |
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Authors: | Bo Young Jeon Soo Jin Kim Dae Hee Kim Byung Kwan Na Doo Hyun Park Hung Thuan Tran Ruihong Zhang Dae Hee Ahn |
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Institution: | (1) Department of Biological Engineering, Seokyeong University, 136-704 Seoul, Korea;(2) Department of Environmental Engineering and Biotechnology, Myongji University, 449-728 Yongin, Korea;(3) Department of Biological and Agricultural Engineering, University of California, Davis, One Shields Avenue, 95616 Davis, CA, USA |
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Abstract: | Aspergillus niger hyphae were found to grow with unliquefied potato starch under aerobic conditions, but did not grow under anaerobic conditions.
The raw culture ofA. niger catalyzed saccharification of potato starch to glucose, producing approximately 12 g glucose/L/day/ The extracellular enzyme
activity was decreased in proportion to incubation time, and approximately 64% of initial activity was maintained after 3
days. At 50°C,A. niger hyphae growth stopped, while the extracellular enzyme activity peaked. On the basis of theA. niger growth property and enzyme activity, we designed a serial bioreactor system composed of four different reactors. Fungal hyphae
were cultivated in reactor I at 30°C, uniquefied starch was saccharified to glycose by a fungal hyphae culture in reactors
II and III at 50°C, and glucose was fermented to ethanol bySaccharomyces cerevisiae in reactor IV. The total glucose produced by fungal hyphae in reactor I and saccharification in reactor II was about 42 g/L/day.
Ethanol production in reactor IV was approximately 22 g/L/day, which corresponds to about 79% of the theoretical maximum produced
from 55 g starch/L/day. |
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Keywords: | serial bioreactor system saccharification Aspergillus niger Saccharomyces cerevisiae ethanol fermentation potato starch |
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