Heparin/polypyrrole (PPy) composite on gold-coated matrix for the neurite outgrowth of PC12 cells by electrical stimulation |
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Authors: | Keun-Hong Park Eun Ae Jo Kun Na |
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Institution: | (1) Cell and Gene Therapy Research Institute, College of Medicine, Pochon CHA University, 135-081 Seoul, Korea;(2) Department of Biotechnology, The Catholic University of Korea, 420-743 Bucheon, Korea |
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Abstract: | A heparin/polypyrrole (PPy) composite, an electrical conducting polymer, was designed to enhance the interactions between
a gold-coated matrix and nerve cells, with the cell (PC12 cells) interactions investigated under different conditions, both
with and without electrical stimulation. The heparin concentration in the composites increased with increasing current density
under the preparation condition, indicating that the heparin concentration in the composite could be controlled by managing
the current density. Optical imaging showed that PC12 cells well attached to the PPy surfaces covered with heparin, but were
poorly interacted to PPy surfaces without the heparin and gold coated matrix. The neurite length of the PC12 cells on the
surfaces with an electrical stimulation (100 mV for 1h) significantly increased, with a median length of 77.5 μm; whereas,
that without electrical stimulation was 10∼20 μm. Therefore, the heparin/polypyrrole (PPy) composite may provide insight for
the development of an ideal nerve guidance channel. |
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Keywords: | electrical conducting polymer heparin neurite outgrowth PC12 cell polypyrrole |
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