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Continuous conversion of rice starch hydrolysate to 2-keto-D-gluconic acid by Arthrobacter globiformis C224 |
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| Authors: | Wen-Hua Teng Wen-Jing Sun Bin Yu Feng-Jie Cui Jing-Ya Qian Jing-Ze Liu Liang Wang Xiang-Hui Qi Hua Wei |
| Institution: | 1. College of Life Science, Hebei Normal University, Shijiazhuang, 050-016, China 2. School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212-013, China 3. Jiangxi Provincial Engineering and Technology Center for Food Additives Bio-production, Dexing, 334-221, China 4. Parchn Sodium Isovitamin C Co., Ltd., Dexing, 334-221, China 5. Jiangxi OAI Joint Research Institute, Nanchang University, Jiangxi, 330-047, China |
| Abstract: | A continuous conversion process of rice starch hydrolysate to 2-keto-D-gluconic acid (2KGA) by Arthrobacter globiformis C224 was developed. Its feasibility for industrial application was also evaluated. Results showed that the initial cell concentration exceeding 1.25 g/L met the continuous 2KGA production at a stable dilution rate and media composition, while the dilution rate and feeding glucose concentration had a significant effect on 2KGA production performance. The optimal operating parameters were obtained as: 0.090/h of dilution rate and 171.0 g/L of feeding glucose concentration. Under these conditions, the steady state had a produced 2KGA concentration of 124.74 g/L, average volumetric productivity of 11.23 g/L/h, and yield of 0.97 g/g. In conclusion, continuous 2KGA production by the A. globiformis C224 strain would be a superior industrial process for the production of 2KGA in terms of its high 2KGA productivity and yield. |
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