|
|||||
|
|
Characterization and evaluation of corn steep liquid in acetone-butanol-ethanol production by Clostridium acetobutylicum |
|
| Authors: | Jin-dal-rae Choi Yu-Sin Jang Jung-Hee Cho Doyoung Seung Sang Yup Lee E Terry Papoutsakis George N Bennett Hyohak Song |
| Institution: | 1619. Biofuel and Biochemical Team, Research and Development Center, GS Caltex Corporation, Daejeon, 305-380, Korea 2619. Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Daejeon, 305-701, Korea 3619. Department of Chemical Engineering and Delaware Biotechnology Institute, University of Delaware, Newark, Delaware, USA 4619. Department of Biochemistry and Cell Biology, Rice University, Houston, Texas, USA |
| Abstract: | Corn steep liquor (CSL) obtained from a commercial starch manufacturing process was analyzed and tested as a complex nutrient source for ABE (acetone, butanol, and ethanol) production by Clostridium acetobutylicum PJC4BK_AdhE1. CSL contained a wealth of trace elements and nitrogenous components, proteins and amino acids. ABE production increased the content of CSL was raised up to 6% (v/v) in medium and then decreased at higher contents. In 6% CSL-containing medium, C. acetobutylicum PJC4BK_AdhE1 produced 21.4 g/L of ABE with a yield of 0.41 g/g in 18 h of fermentation. Although the final titer of ABE was similar in CSL containing media and Clostridial Growth Medium (CGM, containing yeast extract and asparagines as complex nutrients), the yield and productivity of ABE in 6% CSL-containing medium were found to be higher than 32 and 26%, respectively. |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ | |