Optimization of culture conditions and continuous production of chitosan by the fungi,Absidia coerulea |
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Authors: | Woo Jun Kim Woo Gi Lee Kalaimahan Theodore Ho Nam Chang |
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Institution: | (1) Department of Chemical Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, 305-701 Taejon, Korea |
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Abstract: | The production of chitosan from the mycelia ofAbsidia coerulea was studied to improve cell growth and chitosan productivity. Culture conditions were optimized in batch cultivation (pH
4.5 agitator speed of 250 rpm, and aeration rate of, 2 vvm) and the maximum chitosan concentration achieved was 2.3 g/L under
optimized conditions. Continuous culture was carried out successfully by the formation of new growth spots under optimized
conditions, with a chitosan productivity of 0.052 gL−1 h−1, which is the highest value to date, and was obtained at a dilution rate of 0.05 h−1. Cell chitosan concentrations reached about 14% in the steady state, which is similar to that achieved in batch culture.
This study shows that for the continuous culture ofAbsidia coerulea it is vital to control the medium composition. |
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Keywords: | chitosan production Absidia coerulea continuous culture optimum conditions fungal morphology |
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