High frequency plant regeneration of garlic (Allium sativum L.) calli immobilized in calcium alginate gel |
| |
Authors: | Min A Kim Joong Kon Park |
| |
Institution: | (1) Department of Chemical Engineering, Kyungpook National University, 702-701 Daegu, Korea |
| |
Abstract: | Calli obtained from a shoot-tip of garlic,Allium sativum L., were encapsulated using a calcium alginate gel. Some of the encapsulated calli were cultured on a 1/2 MS medium supplemented
with 3% sucrose, 10−5 M kinetin, and 5×10−6 M NAA, whereas the remainder was stored for 40 days at 4°C. All the naked calli regenerated on the solid medium, while 95%
of the encapsulated calli regenerated, and 88% of the encapsulated calli regenerated after 40 days of storage at 4°C. The
capsule matrix delayed the germination time of the encapsulated calli, yet activated the shoot formation of the artificial
garlic seeds. The shoot length of the encapsulated garlic calli was much longer than that of the naked garlic calli. The encapsulated
garlic calli were dried in a laminar airflow cabinet and the conversion frequency of the dried artificial garlic seeds on
a 1/2 MS medium remained at 93% with a water loss of less than 50%. |
| |
Keywords: | artificial seed callus garlic calcium alginate |
本文献已被 SpringerLink 等数据库收录! |
|