Two alleles of the single-copy chalcone synthase gene in parsley differ by a transposon-like element |
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Authors: | Anette Hermann Wolfgang Schulz and Klaus Hahlbrock |
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Institution: | (1) Abteilung Biochemie, Max-Planck-Institut für Züchtungsforschung, D-5000 Köln 30, Federal Republic of Germany |
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Abstract: | We have analysed three nearly full-length cDNAs complementary to mRNAs encoding two PR1 (pathogenesis-related, class 1) proteins in parsley (Petroselinum crispum). Furthermore, one selected genomic clone containing the PcPR1-1 gene was investigated in detail. The structural organization and possible regulatory elements in the 5' flanking region of this gene are presented. In situ RNA hybridization in fungus-infected parsley leaf tissue demonstrated rapid and massive PR1 mRNA accumulation around infection sites. |
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Keywords: | Nucleotide sequence Exon-intron structure UV light activation Flavonoid pathway Petroselinum crispum |
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