A maize cryptic Ac-homologous sequence derived from an Activator transposable element does not transpose. |
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Authors: | Jun-Yi Len Y Henry Sun Yiu-Kay Lai and Jychian Chen |
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Institution: | (1) Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan;(2) Institute of Life Science, Tsing Hua University, Hsinchu, Taiwan |
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Abstract: | Summary Sequences sharing homology to the transposable element Activator (Ac) are prevalent in the maize genome. A cryptic Ac-like DNA, cAc-11, was isolated from the maize inbred line 4Co63 and sequenced. Cryptic Ac-11 has over 90% homology to known Ac sequences and contains an 11 by inverted terminal repeat flanked by an 8 by target site duplication, which are characteristics of Ac and Dissociation (Ds) transposable elements. Unlike the active Ac element, which encodes a transposase, the corresponding sequence in cAc-11 has no significant open reading frame. A 44 by tandem repeat was found at one end of cAc-11, which might be a result of aberrant transposition. The sequence data suggest that cAc-11 may represent a remnant of an Ac or a Ds element. Sequences homologous to cAc-11 can be detected in many maize inbred lines. In contrast to canonical Ac elements, cAc-11 DNA in the maize genome is hypermethylated and does not transpose even in the presence of an active Ac element. |
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Keywords: | Cryptic Ac-homologous sequence Activator transposable element Hypermethylation Transposition Maize |
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