Two isoaccepting seryl tRNAs coded by separate mitochondrial genes in yeast |
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| Authors: | Elisabetta Colletti Laura Frontali Claudio Palleschi Micheline Wesolowski and Hiroshi Fukuhara |
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| Institution: | (1) Centro Acidi Nucleici, Università di Roma, Rome, (Italy);(2) Section de Biologie, Institut Curie, Orsay, France;(3) Istituto di Fisiologia Generale, Facoltà di Scienze M.F.N., Università di Roma, 100185 Roma, (Italy) |
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| Abstract: | Summary In S. cerevisiae four isoacceptor mitochondrial tRNAs for serine have been separated by reversed phase chromatography. At least two of these species are products of different genes. In this work the deletion mapping technique has been used to locate two genes for tRNAser. The gene for tRNAser previously localized in the oli I region of the mitochondrial genome has been found to code for tRNA
ser
2
, and another gene coding for tRNA
ser
1
has been detected in the region where most of other tRNA genes are found. Results of fine mapping experiments allowed to localize this gene in the proximity of the gene for tRNAarg. |
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