Analysis of a Streptomyces antibioticus chromosomal region involved in oleandomycin biosynthesis, which encodes two glycosyltransferases responsible for glycosylation of the macrolactone ring |
| |
Authors: | C Olano A M Rodriguez J-M Michel C Méndez M-C Raynal and J A Salas |
| |
Institution: | (1) Departamento de Biología Funcional, Instituto Universitario de Biotecnologia de Asturias (I.U.B.A-C.S.I.C), Universidad de Oviedo, E-33006 Oviedo, Spain Fax: +34-85-103652, ES;(2) Infectious Disease Group, Hoechst Marion Roussel, 102 Route de Noisy, 93235 Romainville Cedex, France, FR |
| |
Abstract: | A 6-kb region from the chromosome of Streptomyces antibioticus, an oleandomycin producer, was cloned and sequenced. This region was located between the 3′ end of the gene encoding the
third subunit of the oleandomycin type I polyketide synthase and the oleP and oleB genes, which encode a cytochrome P450 monooxygenase and an oleandomycin resistance gene, respectively. Analysis of the nucleotide sequence revealed the presence
of five genes encoding a cytochrome P450-like protein (oleP1), two glycosyltransferases (oleG1 and oleG2) involved in the transfer of the two 6-deoxysugars (L-oleandrose and D-desosamine) to the oleandomycin macrolactone ring,
a methyltransferase (oleM1), and a gene (oleY) of unknown function. Insertional inactivation of this region by gene disruption generated an oleandomycin non-producing
mutant which accumulated a compound that, according to mass spectrometry analysis, could correspond to the oleandomycin macrolactone
ring (oleandolide), suggesting that the mutation affects oleandrosyl glycosyltransferase.
Received: 3 December 1997 / Accepted: 12 May 1998 |
| |
Keywords: | Macrolides Glycosyltransferase Deoxyhexoses Actinomycetes |
本文献已被 SpringerLink 等数据库收录! |
|