Two streptokinase genes are expressed with different solubility in Escherichia coli W3110 |
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| Authors: | Elder Pupo Behnam A Baghbaderani Victoria Lugo Julio Fernández Rolando Páez Isis Torréns |
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| Institution: | (1) Biopharmaceutical Development Division, USA;(2) Pharmaceutics Division, Center for Genetic Engineering and Biotechnology, P.O. Box 6162, Havana, Cuba;(3) Biotechnology Division, Pasteur Institute, Teheran, Iran |
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| Abstract: | The streptokinase (SK) gene from S. equisimilis H46A (ATCC 12449) was cloned in E. coli W3110 under the control of the tryptophan promoter. The recombinant SK, which represented 15% of total cell protein content, was found in the soluble fraction of disrupted cells. The solubility of this SK notably differed from that of the product of the SK gene from S. equisimilis (ATCC 9542) which had been cloned in E. coli W3110 by using similar expression vector and cell growth conditions, and occurred in the form of inclusion bodies. |
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| Keywords: | Escherichia coli purification solubility streptokinase |
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