A versatile assay for total cellulase activity using U-[14C]-labelled bacterial cellulose |
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Authors: | Patricia du Preez A Kistner |
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Institution: | (1) Laboratory for Molecular and Cell Biology, Council for Scientific and Industrial Research, Anaerobic Microbiology Division, Private Bag X3, 0110 ONDERSTEPOORT, South Africa |
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Abstract: | Summary A versatile and sensitive assay for total cellulase activity and not just carboxymethylcellulase activity, is described. The method is equally suitable for studying the kinetics of solubilization of cellulose by growing cells or isolated enzyme fractions. |
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