Isolation and characterization of Acinetobacter sp. ES-1 excreting a lipase with high enantioselectivity for (S)-ketoprofen ethyl ester |
| |
| Authors: | Kwang-Woo Lee Gab-Sang shin Hyun-Ae Bae Hyun-Dong shin and Yong Hyun lee |
| |
| Institution: | (1) Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University, Daegu, 702-701, S. Korea |
| |
| Abstract: | A new Acinetobacter sp. ES-1, grown on triolein, tryptone and Triton X-100, excreted a lipase that hydrolyzed 10m M (R,S)-ketoprofen ethyl ester into (S)-ketoprofen. The crude lipase had an activity of 10Uml-1 and, at 30°C and pH7 over 48h, gave a conversion yield of 35% with an enantiomeric excess for the product 96%. |
| |
| Keywords: | Acinetobacter enantioselective lipase (R S)-ketoprofen ethyl ester (S)-ketoprofen |
| 本文献已被 SpringerLink 等数据库收录！ |
|
