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Development of a novel engineered antibody targeting <Emphasis Type="Italic">Neisseria</Emphasis> species

Authors:	Chih-Yueh Liu Chang-Ching Weng Chih-Hsiang Lin Chiou-Ying Yang Kwok-Kong Tony Mong Yaw-Kuen Li

Institution:	1.Department of Applied Chemistry,National Chiao Tung University,Hsinchu,Taiwan;2.Institute of Molecular Biology,National Chung Hsing University,Taichung,Taiwan

Abstract:	Objectives A Neissaria bacterial pilus sugar, bacillosamine, was synthesized and, for the first time, used as a probe to screen a single-chain variable fragment (scFv). Results Four Neisseria, Neisseria gonorrhoeae, Neisseria meningitidis, Neisseria sicca and Neisseria subflava, and two negative controls, Streptococcus pneumoniae and Escherichia coli, were tested through ELISA, immunostaining and gold nanoparticle immunological assay. All results indicated that the selected scFv is feasible for the specific detection of Neisseria species via the recognition of bacillosamine. Conclusions The recombinant scFv could detect Neisseria strains at 10⁶ CFU/ml.

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