Isolation of human periosteum-derived progenitor cells using
immunophenotypes for chondrogenesis |
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Authors: | Sang?Min?Lim Yong?Soo?Choi Hyun?Chong?Shin Chang?Woo?Lee Email author" target="_blank">Dong-Il?KimEmail author |
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Institution: | (1) Department of Biological Engineering, Inha University, Incheon, 402-751, Korea;(2) Good Shepherd Hospital, Yeoksam-dong, Seoul, 135-717, Korea |
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Abstract: | Periosteum-derived progenitor cells (PDPCs) were isolated by characteristic surface markers. Reproducibility of immunophenotypes of the PDPCs was characterized by flow cytometric analysis using fluorescence-activated cell sorter (FACS). SH2+, SH3+, SH4+, CD9+, CD90+ and CD105+ were important eternal characteristic cell surface markers for the PDPCs. The characterized PDPCs maintained their chondrogenic potential in pellet cultures until the 15th passage from primary cell culture. |
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Keywords: | chondrogenesis immunophenotype periosteum-derived cells |
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