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Isolation of human periosteum-derived progenitor cells using immunophenotypes for chondrogenesis
Authors:Sang?Min?Lim  Yong?Soo?Choi  Hyun?Chong?Shin  Chang?Woo?Lee  Email author" target="_blank">Dong-Il?KimEmail author
Institution:(1) Department of Biological Engineering, Inha University, Incheon, 402-751, Korea;(2) Good Shepherd Hospital, Yeoksam-dong, Seoul, 135-717, Korea
Abstract:Periosteum-derived progenitor cells (PDPCs) were isolated by characteristic surface markers. Reproducibility of immunophenotypes of the PDPCs was characterized by flow cytometric analysis using fluorescence-activated cell sorter (FACS). SH2+, SH3+, SH4+, CD9+, CD90+ and CD105+ were important eternal characteristic cell surface markers for the PDPCs. The characterized PDPCs maintained their chondrogenic potential in pellet cultures until the 15th passage from primary cell culture.
Keywords:chondrogenesis  immunophenotype  periosteum-derived cells
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