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Enhanced paclitaxel production induced by the combination of elicitors in cell suspension cultures of Taxus chinensis
Authors:CH Zhang  XG Mei  L Liu  LJ Yu
Institution:(1) School of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China
Abstract:Taxus chinensis suspension cells were cultured in the modified Gamborg's B5 medium. Addition of 50 mg chitosan l–1, 60 mgrM methyl jasmonate and 30 mgrM Ag+ resulted in the greatest paclitaxel production, at 25 mg l–1 in the cultures, being almost 40 times higher than that of the control culture, 10 times higher than that of the culture exposed to Ag+, 6 times higher than that of the culture elicited by chitosan and almost double that of the culture elicited by methyl jasmonate.
Keywords:combined-elicitation  elicitor  paclitaxel (taxol)  Taxus chinensis
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