Enhanced paclitaxel production induced by the combination of elicitors in cell suspension cultures of Taxus chinensis |
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Authors: | CH Zhang XG Mei L Liu LJ Yu |
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Institution: | (1) School of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China |
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Abstract: | Taxus chinensis suspension cells were cultured in the modified Gamborg's B5 medium. Addition of 50 mg chitosan l–1, 60 M methyl jasmonate and 30 M Ag+ resulted in the greatest paclitaxel production, at 25 mg l–1 in the cultures, being almost 40 times higher than that of the control culture, 10 times higher than that of the culture exposed to Ag+, 6 times higher than that of the culture elicited by chitosan and almost double that of the culture elicited by methyl jasmonate. |
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Keywords: | combined-elicitation elicitor paclitaxel (taxol) Taxus chinensis |
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