Expression and secretion of human lipocortin-1 by promoter and signal sequence of STA1 from Saccharomyces diastaticus |
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Authors: | Soo Wan Nam Byung Moon Kim Bong Hyun Chung Dae Ook Kang Jong Seog Ahn |
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Institution: | (1) Genetic Engineering Research Institute, KIST, P.O. Box 115, 305-600 Yusong, Taejon, Korea |
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Abstract: | Summary For the secretion of human lipocortin-1 (LC-1) in yeast, a expression and secretion vector was constructed by using the promoter and signal sequence of glucoamylase gene (STA1) of Saccharomyces diastaticus. After the cDNA of human LC-1 was ligated with the secretion vector, the resulting hybrid plasmid was transformed into S. diastaticus. When the recombinant S. diastaticus was cultivated in YPD medium, LC-1 was expressed and secreted into the extracellular medium, yielding LC-1 protein at a concentration of 2.5 g/mL. |
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