Recombinant expression of human cathelicidin (hCAP18/LL-37) in <Emphasis Type="Italic">Pichia pastoris</Emphasis> |
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Authors: | In-Pyo Hong Sung-Jae Lee Yong-Seok Kim Shin-Geon Choi |
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Institution: | (1) Department of Bioengineering and Technology, Kangwon National University, 192-1, Hyoja2-dong, Chuncheon, 200-701, Korea;(2) Department of Biochemistry and Molecular Biology, College of Medicine, Hanyang University, 17 Haengdang-dong, Seongdong-gu, Seoul, 133-791, Korea |
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Abstract: | The constitutive expression of human cathelicidin LL-37 antimicrobial peptide was achieved using the methylotrophic yeast,
Pichia pastoris. An LL-37 cDNA clone was amplified by PCR using human fetal cDNA library as template. The 111 bp fragment encoding mature
LL-37 gene was subcloned into pGAPZ-E, an episomal form of the pGAPZB vector incorporating PARS1. It was then transformed
into the P. pastoris X-33 strain for intracellular expression. A small peptide with a molecular mass of about 5 kDa was detected by 17% peptide-PAGE
analysis. The recombinant LL-37 peptide was purified from the gel and its amino acid sequence was determined by LC-ESI-MS/MS
analysis. The initiating amino acid, methionine, was still attached to the N-terminal region of recombinant LL-37. LL-37 crude extract from P. pastoris showed an antimicrobial activity against Micrococcus luteus as the test strain. The successful expression of human LL-37 indicates that the system may be applicable to the expression
of other human defensins without resorting to fusion protein constructions. |
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Keywords: | Cathelicidin LL-37 Heterologous expression Pichia pastoris |
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