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Decreased peroxidase activity in transgenic tobacco and its effect on lignification |
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| Authors: | Tijen Talas-Oğraş Kemal Kazan Nermin Gözükırmızı |
| Institution: | (1) Marmara Research Center, Research Institute for Genetic Engineering and Biotechnology, TUBITAK, 41470 Gebze, Kocaeli, Turkey;(2) Cooperative Research Center for Tropical Plant Pathology, University of Queensland, 4072 Brisbane, Australia;(3) Department of Biology, Faculty of Science, University of Istanbul, 34459 Istanbul, Turkey |
| Abstract: | An antisense gene construct of a peroxidase gene (Shpx6a) from a tropical pasture legume Stylosanthes humilis was transferred into tobacco cells via Agrobacterium tumefaciens to test whether peroxidase activity could be decreased and what effect this would have on lignification. A large number of tobacco cell lines were regenerated on selective media and stable integration of the transgene was confirmed in randomly selected putative transformants. Analyses of the primary transgenic plants and their progeny (T 1) demonstrated that the total peroxidase activity was significantly decreased (up to 36%) as compared to that measured in untransformed control plants. Importantly, reduction in peroxidase activity is accompanied by decreases (up to 23%) in lignin content in several transgenic lines. |
| Keywords: | antisense expression lignification peroxidase |
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