Metabolic engineering of <Emphasis Type="Italic">Saccharomyces cerevisiae</Emphasis> for linalool production |
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| Authors: | Pegah Amiri Azar Shahpiri Mohammad Ali Asadollahi Fariborz Momenbeik Siavash Partow |
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| Institution: | 1.Department of Agricultural Biotechnology, College of Agriculture,Isfahan University of Technology,Isfahan,Iran;2.Department of Biotechnology, Faculty of Advanced Sciences and Technologies,University of Isfahan,Isfahan,Iran;3.Department of Analytical Chemistry, Faculty of Chemistry,University of Isfahan,Isfahan,Iran;4.Department of Chemical Engineering & Applied Chemistry,University of Toronto,Toronto,Canada |
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| Abstract: | ObjectivesTo engineer the yeast Saccharomyces cerevisiae for the heterologous production of linalool.ResultsExpression of linalool synthase gene from Lavandula angustifolia enabled heterologous production of linalool in S. cerevisiae. Downregulation of ERG9 gene, that encodes squalene synthase, by replacing its native promoter with the repressible MET3 promoter in the presence of methionine resulted in accumulation of 78 µg linalool l?1 in the culture medium. This was more than twice that produced by the control strain. The highest linalool titer was obtained by combined repression of ERG9 and overexpression of tHMG1. The yeast strain harboring both modifications produced 95 μg linalool l?1.ConclusionsAlthough overexpression of tHMG1 and downregulation of ERG9 enhanced linalool titers threefold in the engineered yeast strain, alleviating linalool toxicity is necessary for further improvement of linalool biosynthesis in yeast. |
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