Ca2+ and Cu2+ supplementation augments vancomycin production by Amycolatopsis orientalis |
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Authors: | Hyung-Moo Jung Sang-Yong Kim Hyung-Hwan Hyun Jung-Kul Lee |
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Institution: | (1) BioNgene Co., Ltd., Chongro-Ku, Seoul, 110-521, Korea;(2) Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies, 89 Wangsan-ri, Mohyun, Yongin-si, Kyounggi-do, 449-791, Korea |
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Abstract: | Supplementation with CaCl22H2O (40 mg l–1) or CuSO45H2O (10 mg l–1) improved vancomycin production by Amycolatopsis orientalis by 12 and 11%, respectively; used in combination, they acted synergistically. Ca2+ decreased the intracellular concentration of vancomycin 36%, whereas Cu2+ increased the intracellular activity of TDP-glucose:aglycosylvancomycin glucosyltransferase 3 times more than control. Ca2+ probably works by altering the permeability of cells to vancomycin, whereas Cu2+ increases the activity of an enzyme responsible for vancomycin biosynthesis. |
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Keywords: | Amycolatopsis orientalis calcium copper vancomycin |
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