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Optimization of conditions for the production of pullulan and high molecular weight pullulan by Aureobasidium pullulans |
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| Authors: | Ji-Hyun Lee Jeong-Hwa Kim Il-Hui Zhu Xiao-Bei Zhan Jin-Woo Lee Dong-Hoon Shin Sung-Koo Kim |
| Institution: | (1) Division of Food and Biotechnology, Pukyung National University, Pusan, 608-737, Korea;(2) Division of Biotechnology, Wuxi University of Light Industry, Wuxi, 214036, China;(3) Faculty of Natural Resources & Life Science, Dong-A University, Pusan, 604-714, Korea;(4) Graduate School of Biotechnology, Korea University, Seoul, 136-701, Korea |
| Abstract: | Aureobasidium pullulans had a maximum yield coefficient of pullulan (Y p/s=0.24) with an initial pH of the culture broth of 6.5 in a shake-flask culture. In a batch culture, the maximum pullulan yield coefficient of 0.30 was obtained at the aeration rate of 0.5 vvm. A yeast-like form and mycelial form of cells were found at the culture broth with pH controlled at 4.5 with a maximum yield coefficient of pullulan of 0.27. However, a high portion (35%) of high molecular weight pullulan (M w>2 000 000) was produced at pH 6.5 with a yeast-like morphology of the cells. |
| Keywords: | molecular weight pH pullulan |
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